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Bone development in offspring of pregnant rats treated with carbamazepine: Evaluation by three different methods

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dc.contributor.author Guler, Hatice
dc.contributor.author Esen, Eda Esra
dc.contributor.author Balcioglu, Esra
dc.contributor.author Goktepe, Ozge
dc.contributor.author Yilmaz, Halil
dc.contributor.author Yay, Arzu Hanim
dc.contributor.author Nisari, Mehtap
dc.contributor.author Al, Ozge
dc.contributor.author Ucar, Sumeyye
dc.contributor.author Ekinci, Hilal Kubra Guclu
dc.contributor.author Tokpinar, Adem
dc.contributor.author Yilmaz, Seher
dc.date.accessioned 2024-03-26T06:32:28Z
dc.date.available 2024-03-26T06:32:28Z
dc.date.issued 2022
dc.identifier.citation Güler, H., Esen, EE., Balcioglu, E., Göktepe, Ö., Yilmaz, H., Yay, AH., Nisari, M., Al, Ö., Uçar, S., Ekinci, HKG., Tokpinar, A., Yilmaz, S. (2022). Bone development in offspring of pregnant rats treated with carbamazepine: Evaluation by three different methods. Epilepsia, 63(12), 3066-3077. https://doi.org/10.1111/epi.17422 en_US
dc.identifier.issn 0013-9580
dc.identifier.issn 1528-1167
dc.identifier.uri http://dx.doi.org/10.1111/epi.17422
dc.identifier.uri https://www.webofscience.com/wos/woscc/full-record/WOS:000865815900001
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/5078
dc.description WoS Categories: Clinical Neurology en_US
dc.description Web of Science Index: Science Citation Index Expanded (SCI-EXPANDED) en_US
dc.description Research Areas: Neurosciences & Neurology en_US
dc.description.abstract Objective This study was carried out to determine the effect of intrauterine carbamazepine (CBZ) exposure on fetal bone development during pregnancy. Methods In the study, 24 female Wistar pregnant rats were used. Rats were 20 weeks old. They had an average body weight of 150-200 g. Pregnant rats were randomly selected and divided (n = 6) into a control group, low-dose CBZ (10 mg/kg/day) group, medium-dose CBZ (25 mg/kg/day) group, and high-dose CBZ (50 mg/kg/day) group. The ossification length (mm) and ossification area (mm(2)) of the long bones of the fetuses in the experimental and control groups were calculated. The densities of alkaline phosphatase (AP) and tartrate-resistant acid phosphatase (TRAP) were analyzed. The ossification regions of the femurs of the fetuses were examined under a light microscope. Microstructural images of the femurs were evaluated with scanning electron microscope photographs. The densities of minerals involved in the ossification process were analyzed. Results According to the results of the study, all three doses of CBZ caused loss of ossification areas, and it was observed that this bone loss also increased statistically significantly depending on the dose increase (p < .05). Calcium concentration decreased in the CBZ groups. When the electron microscope images were examined, it was determined that the cartilage matrix of the CBZ groups was thinned. In the histological evaluation of the groups, narrowing of the primary bone collar and smaller bone spicules in the ossification region compared to the control group were noted due to the increase in dose in the CBZ groups. In immunohistochemical staining, it was observed that the TRAP and AP expression values of the femurs were the lowest in the CBZ groups. These decreases were also statistically significant when compared with the control group. Significance It was revealed with both microscopic and macroscopic findings that exposure to intrauterine CBZ negatively affected ossification and bone growth. en_US
dc.description.sponsorship Erciyes University Scientific Research Projects Coordination Unit [TKB-2020-9988] en_US
dc.language.iso eng en_US
dc.publisher WILEY-HOBOKEN en_US
dc.relation.isversionof 10.1111/epi.17422 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject bone development, carbamazepine, immunohistochemistry staining, pregnancy, scanning electron microscopy en_US
dc.subject ANTIEPILEPTIC DRUGS, MAJOR MALFORMATIONS, PRENATAL EXPOSURE, VALPROIC ACID, VITAMIN-D, EPILEPSY, CHILDREN, INFANTS, METABOLISM, TURNOVER en_US
dc.title Bone development in offspring of pregnant rats treated with carbamazepine: Evaluation by three different methods en_US
dc.type article en_US
dc.relation.journal EPILEPSIA en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0003-3378-3745 en_US
dc.contributor.authorID 0000-0002-8234-4901 en_US
dc.contributor.authorID 0000-0002-1126-7478 en_US
dc.contributor.authorID 0000-0001-7849-1768 en_US
dc.contributor.authorID 0000-0002-0541-8372 en_US
dc.contributor.authorID 0000-0001-5292-3593 en_US
dc.contributor.authorID 0000-0001-9364-5948 en_US
dc.identifier.volume 63 en_US
dc.identifier.issue 12 en_US
dc.identifier.startpage 3066 en_US
dc.identifier.endpage 3077 en_US


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