Evaluation of Lethal and Sublethal Effects of Methyl Benzoate on the Generalist Predator Orius laevigatus (Fieber)

Abstract

Methyl benzoate (MBe), a volatile organic molecule, has been shown to have insecticidal effects on a variety of agricultural, stored products, and urban arthropod pests in recent investigations. However, the toxicity of MBe against nontarget organisms has rarely been investigated. This study investigated the lethal and sublethal effects of MBe on the generalist predator Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) via different exposure routes. This species is an important natural enemy of thrips, aphids, and mites in biological control programs globally. Acute toxicity bioassays conducted on O. laevigatus showed that the lethal median concentration (LC50) values of MBe for topical and residual toxicity were 0.73 and 0.94%, respectively, after 24 hr of exposure. Importantly, a sublethal concentration of MBe (LC30 = 0.51%) did not affect the survival and reproduction of O. laevigatus. In addition, prey consumption by O. laevigatus under different exposure conditions with varying densities of Aphis gossypii (Glover) (Hemiptera: Aphididae) adults demonstrated a good fit for a Type II functional response. The sublethal concentration of MBe did not affect the attack rate and handling time of O. laevigatus compared to untreated insects, nor did it affect the longevity and fecundity of O. laevigatus females. Thus, according to the International Organization for Biological Control, the sublethal MBe concentration for O. laevigatus is categorized as harmless and may be used in conjunction with this predator species for integrated control of many agricultural insect pests.