Designing functional materials: DNA/Poly(3,4-ethylenedioxythiophene) interfaces for advanced DNA direct electrochemistry and DNA-Drug interaction detection

Abstract

In the present study, preparation of poly(3,4-ethylenedioxythiophene) coated pencil graphite electrode with the addition of multi-walled carbon nanotubes (MWCNTs) (PEDOT nanoelectrode, NE); incorporation of double-stranded deoxyribonucleic acid (dsDNA) onto NE (dsDNA/PEDOT NE); and the bio-application of dsDNA/PEDOT NE were described. PEDOT NEs were first used to immobilize dsDNA onto the electrode surfaces in a high efficiency to detect dsDNA with advanced oxidation signals. Afterwards, these functional biointerfaces were used for the investigation of DNA interaction with Mitomycin C (MC) and Paclitaxel (PTX) based on guanine and adenine oxidation signals. dsDNA/PEDOT NE was presented a linearity in the concentration of 1 mg L-1 to 50 mg L-1 for MC with a detection limit of 0.26 mg L-1 . The linear range was between 0.5 mg L-1 and 60 mg L-1 for PTX with a detection limit of 0.14 mg L-1 (n = 3). In addition, dsDNA/PEDOT NE was presented good stability.