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Occurency of Giardia duodenalis assemblages in river water sources of Black Sea, Turkey

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dc.contributor.author Karanis, Panagiotis
dc.contributor.author Koloren, Zeynep
dc.contributor.author Seferoglu, Onuralp
dc.date.accessioned 2022-08-19T12:29:19Z
dc.date.available 2022-08-19T12:29:19Z
dc.date.issued 2016
dc.identifier.uri http://doi.org/10.1016/j.actatropica.2016.09.025
dc.identifier.uri https://www.sciencedirect.com/science/article/pii/S0001706X16305575?via%3Dihub
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3004
dc.description.abstract A total of 420 environmental water samples and 120 drinking water samples from 45 different sampling sites of the Black Sea in Turkey were collected between 2012 and 2014. Genomic DNA was isolated from all the investigated water samples and comparativelly analyzed by Loop-mediated isothermal amplification (LAMP) of the elongation factor 1 Alfa (EF1 alpha) gene, and by nested Polymerase Chain Reaction (nPCR) of the small subunit (SSU) rRNA and semi-nested PCR (snPCR) of the glutamate dehydrogenase gene (GDH). 141 (58.7%), 125 (52.1%) and 120 (50%) samples respectivelly were positive by each method. Out of 240 environmental samples collected from 25 sites of Samsun Province have been found positive for G. duodenalis by LAMP, nPCR and snPCR, respectively. 55 (30.5%), 50 (27.8%) and 47 (26.1%) of 180 environmental samples collected from 20 other sampling sites of Giresun Province were positive for Giardia by LAMP, nPCR and snPCR, respectively. Five PCR products from different samples of the Giresun Province and 10 other samples from the Samsun Province were found positive for G. duodenalis assemblage B. Five PCR products from Giresun Province and 5 samples from Samsun Province were found positive for G. duodenalis assemblage A. This is the first report about G. duodenalis assemblages A and B from water samples investigations in Black Sea of Turkey. (C) 2016 Elsevier B.V. All rights reserved. en_US
dc.language.iso eng en_US
dc.publisher ELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS en_US
dc.relation.isversionof 10.1016/j.actatropica.2016.09.025 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Giardia duodenalis; PCR; LAMP; Assemblages A and B; River water; Giresun; Samsun; Turkey en_US
dc.subject WASTE-WATER; MOLECULAR CHARACTERIZATION; PROTOZOAN PARASITES; NESTED PCR; CRYPTOSPORIDIUM; GENOTYPES; TRANSMISSION; SUPPLIES; IDENTIFICATION; EPIDEMIOLOGY en_US
dc.title Occurency of Giardia duodenalis assemblages in river water sources of Black Sea, Turkey en_US
dc.type article en_US
dc.relation.journal ACTA TROPICA en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0001-9708-2716 en_US
dc.contributor.authorID 0000-0002-0733-0470 en_US
dc.identifier.volume 164 en_US
dc.identifier.startpage 337 en_US
dc.identifier.endpage 344 en_US


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