Morphology, histology and phylogeny of Henneguya sinova sp nov (Myxobolidae: Myxozoa) infecting gills of Parablennius tentacularis in the Black Sea, Turkey

Abstract

Myxosporeans of the genus Henneguya have a global distribution and infect organs and tissues of both marine and freshwater fishes. Here we describe the morphological, histological and molecular characteristics of Henneguya sinova sp. nov. parasitizing the gill arches of tentacled blenny Parablennius tentacularis (Perciformes: Blenniidae) collected from the coast of Sinop on the Black Sea in Turkey. Several oval whitish plasmodia of different sizes in the gill arches of fish were found. The mature spores were rounded oval in frontal view, with a mean (range) total length 57.5 (51.5-68.0) mu m; the spore body was 11.7 (11.3-12.0) mu m in length by 7.6 (7.3-8.3) mu m in width and 6.7 (6.6-6.8) mu m in thickness. The caudal appendages, measuring 46.0 (40.0-55.0) mu m in length, were very thin at the tapered end. The prevalence of infection by H. sinova sp. nov. was 35.5%. Phylo genetic analysis of nuclear small subunit ribosomal DNA (SSU rDNA) clearly suggested H. sinova as a new species which is clustered within the marine Henneguya lineage. Pairwise nucleotide similarities and DNA distance values of SSU rDNA between H. sinova sp. nov. and other related Henneguya species also supported this suggestion.