Biostimulatory Effects of Diode Laser and Ozone on Wound Healing in Rats

Abstract

Objective: Diode laser and ozone can be used to healing of skin wounds. The aim of this study is to evaluate the biostimulatory effects of diode laser and ozone on the healing of sutured skin wounds in rats.

Materials and Methods: In this study, 21 adult males Wistar albino rats were used. Three 1-cm-longs, full-thickness incisions were applied to the back of each animal and closed with sutures. Diode laser treatment, irrigation with 0.9% saline solution and ozone therapy was applied to the wounds through 7 days. Animals were euthanized on the 3rd, 7th, and 14th days after treatment, and all wounds were surgically removed for histopathological evaluation.

Results: According to histological results, a statistically significant difference was found among the three treatment methods in acute inflammatory reactions on 3rd day (p<0.05). Ozone treatment reduced the intensity of acute inflammatory reactions by the 3rd day (p<0.05). In addition, a statistically significant difference was found between the follow-up periods in all treatment groups for fibrosis (p<0.01).

Conclusion: It was found that ozone treatment was more effective when compared to the other treatments for reducing the intensity of acute inflammatory reactions on the healing of sutured skin wounds.