MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF LOCAL COMMON BEAN (PHASEOLUS VULGARIS L.) GENOTYPES

Abstract

This study was conducted for morphological and molecular characterization of 33 common bean genotypes collected from Ordu province in Turkey. Genetic relationships among the local common bean genotypes were also identified. In presented genotypes, 3 phenological and 22 morphological characteristics were investigated. The first flower and the first beans were formed 43.42 and 48.55 days after sowing time respectively. The first fresh bean harvest was realized 67.85 days after the sowing time. Pod lengths and pod widths of the genotypes varied between 10.93 and 23.23 cm (average 14.76 cm) and 9.39-22.73 mm (average 15.22 mm) respectively. The first 3 dimensions (PC1, PC2 and PC3) of principal component analysis on morphological data explained 72.27% of total variation. Seed main color, seed secondary color, pod color, pod cross-section and stringiness were prominent characteristics in screening of common bean genotypes. Of the SSR primers, SSR-IAC116 yielded the greatest PIC value (0.82) and it was followed by BMD-45-AIA (0.77) and PV ag004 (0.72) primers. The primers had a mean number of alleles per locus of 2.55. The BM210 had the greatest number of polymorphic alleles per locus (6 alleles). Cluster analysis, composed from molecular data, revealed 3 main groups. The genotypes G14 and G17 were placed alone in the first and second groups and the rest were clustered in the third group. The similarity index values among 33 local common bean genotypes varied between 0.34 and 0.97. The genotypes G04 and G22 were identified as the closest genotypes with a genetic similarity coefficient of 0.97.