Shoot Organogenesis From Leaf Discs in Some Quince (Cydonia oblonga Mill.) Genotypes

Abstract

The objective of this study was to stimulate organogenesis of in vitro shoots from leaf discs in some quince (Cydonia oblonga Mill.) genotypes. Quince A (dwarf), S.O. (Sabahattin Ozbek) 39-200 (dwarf), S.O. 18-82 (semi dwarf) and S.O. 58-315 (vigorous) clonal rootstocks, and E me, Limon, and Cukurgobek cultivars were used as plant materials. Effects of TDZ + NAA combinations or BA + NAA, AgNO3 and putrescine treatments were investigated in two experiments for organogenesis. Shoot organogenesis was significantly induced on MS (Murashige and Skoog) basal medium containing 0.33 mg/l TDZ + 0.5mg/l NAA + 2.0 mg/l AgNO3. The rate of shoot formation was 80.0% in Quince A, 56.7% in S.O. 39-200, 17.5% in S.O. 18-82, 37.5% in S.O. 58-315, 36.7% in E me, 40.0% in Limon, and 43.3% in Cukurgobek. The average shoot number per leaf disc was changed between 0.4 and 2.7 among genotypes.