1. Ordu Üniversitesi Açık Arşiv Sistemi
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  3. Tıp Fakültesi
  4. Temel Tıp Bilimleri
Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3591
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dc.contributor.authorSaglam, Tugba-
dc.contributor.authorDusen, Serdar-
dc.contributor.authorMete, Ergun-
dc.contributor.authorKaraman, Ulku-
dc.date.accessioned2023-01-06T12:00:25Z-
dc.date.available2023-01-06T12:00:25Z-
dc.date.issued2022-
dc.identifier.citationSaglam, T., Dusen, S., Mete, E., Karaman, U. (2022). Comparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkey. Acta Parasitologica, 67(1), 555-559.Doi:10.1007/s11686-021-00494-1en_US
dc.identifier.isbn1230-2821-
dc.identifier.isbn1896-1851-
dc.identifier.urihttp://dx.doi.org/10.1007/s11686-021-00494-1-
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000722115300001-
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/34817741-
dc.identifier.urihttp://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3591-
dc.descriptionWoS Categories : Parasitology; Veterinary Sciences; Zoology Web of Science Index : Science Citation Index Expanded (SCI-EXPANDED) Research Areas : Parasitology; Veterinary Sciences; Zoology Open Access Designations : Green Published, Bronzeen_US
dc.description.abstractPurpose While Toxoplasma gondii (T. gondii) infection is asymptomatic in immunocompetent individuals, it is a life-threatening protozoan in immunocompromised individuals. Its water-borne transmission to humans poses a serious public health concern. Polymerase Chain Reaction (PCR) has a considerable potential for the sensitive and specific detection of T. gondii oocysts in waters. Methods Comparative evaluation of RE 529-bp sequence and B1 gene to detect T. gondii tachyzoites and oocysts via PCR in agricultural irrigation water taken from downtown Denizli, Turkey and water samples collected from neighborhood fountains was performed for the first time in Turkish context. Results Based on real-time PCR targeting the B1 genetic markers and RE 529-bp sequence, T. gondii DNA was identified in 6 (16.7%) out of 48 samples collected from agricultural irrigation water. Besides, our PCR analysis did not establish any presence of T. gondii in drinking water samples. Conclusion T. gondii showed lower sensitivity in B1-based PCR than in PCR targeting RE 529-bp sequence.en_US
dc.description.sponsorshipFunding Orgs : Pamukkale University Scientific Research Projects Unit Funding Name Preferred : Pamukkale University Scientific Research Projects Unit(Pamukkale University) Funding Text : Pamukkale University Scientific Research Projects Unit.en_US
dc.language.isoengen_US
dc.publisherSPRINGER INT PUBL AG CHAMen_US
dc.relation.isversionof10.1007/s11686-021-00494-1en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTIME PCR; OOCYSTS; CRYPTOSPORIDIUM; PURIFICATION; INFECTIONen_US
dc.subjectToxoplasma gondii; 529-bp; B1 gene; Water-borne; Denizli; Turkeyen_US
dc.titleComparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkeyen_US
dc.typearticleen_US
dc.relation.journalACTA PARASITOLOGICAen_US
dc.contributor.departmentOrdu Üniversitesien_US
dc.contributor.authorID0000-0003-1654-2261en_US
dc.identifier.volume67en_US
dc.identifier.issue1en_US
dc.identifier.startpage555en_US
dc.identifier.endpage559en_US
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