Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3576
Title: Electrochemical catechol biosensor based on beta-cyclodextrin capped gold nanoparticles and inhibition effect of ibuprofen
Authors: Kapan, Beste
Kurbanoglu, Sevinc
Esenturk, Emren Nalbant
Soylemez, Saniye
Toppare, Levent
Ordu Üniversitesi
0000-0002-8955-133X
0000-0002-4337-3312
Keywords: ENZYME-INHIBITION; SILVER NANOPARTICLES; IMMOBILIZATION; GRAPHENE; EQUATION; SENSOR; FOOD
Catechol detection; Enzyme inhibition; beta cyclodextrin; Gold nanoparticles; Ibuprofen; Tyrosinase
Issue Date: 2021
Publisher: ELSEVIER SCI LTD OXFORD
Citation: Kapan, B., Kurbanoglu, S., Esenturk, EN., Soylemez, S., Toppare, L. (2021). Electrochemical catechol biosensor based on beta-cyclodextrin capped gold nanoparticles and inhibition effect of ibuprofen. Process Biochemistry, 108, 80-89.Doi:10.1016/j.procbio.2021.06.004
Abstract: Herein, beta-cyclodextrin-capped gold nanoparticle surface designed for tymsinase (Tyr)-based nanosensor is proposed and demonstrated. Integration of the beta-cyclodextrin-capped gold nanoparticles on graphite electrode surface was achieved via drop-casting method, Tyr was immobilized on the modified electrode and then used as drug inhibition platform with catechol as the substrate. All optimization studies that affect biosensor response were conducted, and catechol was detected in the linear range of 1.56 mu M-25 mu M catechol concentration with a limit of detection of 0.42 mu M and sensitivity of 2.094 mu A.mu M-1.cm(-2).Tyr inhibition was followed with ibuprofen drug active compound with a 15 min incubation time, and the I-50 value was found as 213 mu M. The sensor is the first in the literature to use an electrochemical method for beta-cyclodextrin-capped gold nanoparticles based Tyr biosensor and ibuprofen inhibition. This sensor presents an easy fabrication method, excellent sensor properties, and ibuprofen inhibition capabilities. As a result, it is proposed that the designed sensor is an ideal marker for detecting Tyr inhibition using electrochemical methods and an ibuprofen-based cosmetic cream formulation could be a viable option in the cosmetic market.
Description: WoS Categories : Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical Web of Science Index : Science Citation Index Expanded (SCI-EXPANDED) Research Areas : Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
URI: http://dx.doi.org/10.1016/j.procbio.2021.06.004
https://www.webofscience.com/wos/woscc/full-record/WOS:000677490400009
http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3576
ISBN: 1359-5113
1873-3298
Appears in Collections:Kimya

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