Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1352
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dc.contributor.authorAygun, Ahmet-
dc.contributor.authorDumanoglu, Hatice-
dc.date.accessioned2022-08-15T10:51:32Z-
dc.date.available2022-08-15T10:51:32Z-
dc.date.issued2015-
dc.identifier.urihttp://doi.org/10.3389/fpls.2015.00225-
dc.identifier.urihttp://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1352-
dc.description.abstractShoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest percentages (>80%) of shoot proliferation were obtained in the mediums supplemented with 9.0 mu M BA and 0.5 mu M indole-3-acetic acid. In the subcultures, the highest shoot proliferation rates were obtained in the medium containing 4.5 and 9.0 FM BA. The shoot proliferation rates ranged from 91.1 +/- 2.4 to 96.4 +/- 2.0% in the second subculture and from 76.7 +/- 7.8 to 89.4 +/- 3.3% in the third subculture. In the second subculture, the shoots grown on 9.0 mu M BA without auxin produced the best proliferation (10.6 +/- 1.6). For the in vitro rooting experiments, the highest rooting rate (54.2 +/- 10.4%), root length (10.5 +/- 2.4 mm), and root number (2.5 +/- 0.6) were obtained from 10 days dark treatment on the medium containing half strength of macronutrients supplemented with 5 mu M indole-3-butyric acid (IBA). For the ex vitro rooting experiments, shoot rooting was significantly influenced by 10 mM IBA applied as quick-dip method. The percentage of rooting was 55 +/- 9.6% and root number was 1.8 +/- 0.3 at this concentration.en_US
dc.language.isoengen_US
dc.publisherFRONTIERS MEDIA SAAVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE CH-1015, SWITZERLANDen_US
dc.relation.isversionof10.3389/fpls.2015.00225en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmicropropagation, Pyrus elaeagrifolia, tissue culture, wild pearen_US
dc.titleIn vitro shoot proliferation and in vitro and ex vitro root formation of Pyrus elaeagrifolia Pallasen_US
dc.typearticleen_US
dc.relation.journalFRONTIERS IN PLANT SCIENCEen_US
dc.contributor.departmentOrdu Üniversitesien_US
dc.contributor.authorID0000-0001-7745-3380en_US
dc.contributor.authorID0000-0002-7099-7630en_US
dc.identifier.volume6en_US
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