Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1311
Title: Molecular identification of root-knot nematode Meloidogyne incognita from kiwi fruit orchards in Ordu province, Turkey
Authors: Akyazi, Faruk
Felek, Anil Firat
Ordu Üniversitesi
0000-0002-5239-2849
0000-0002-3603-7046
Keywords: Actinidia deliciosa Kiwi Meloidogyne incognitar DNA Root-knot nematodes SCAR
Issue Date: 2013
Publisher: ENTOMOLOGICAL SOC TURKEY, EGE UNIVZIRAAT FAKULTESI, BITKI KORUMA BOLUMU, BORNOVA IZMIR 00000, TURKEY
Abstract: Root-knot nematodes (Meloidogyne spp.) are one of the major groups of nematodes identified on kiwi fruit (Actinidia deliciosa Chev.). This investigation was conducted to identify root-knot nematodes encountered the in kiwi fruit orchards in the Ordu province of Turkey by means of Polymerase Chain Reaction (PCR). For this purpose, infected plant root samples were collected from 17 kiwi fruit orchards located in Center, Persembe, Unye, Gulyali, Ikizce and Ulubey districts of the province during the years 2011-2012. Females directly obtained from the infected roots of orchard populations were used for the molecular identifications. It was carried out with an identification key using ribosomal DNA (rDNA) region, Intergenic Spacer (IGS-2) region between 5S-18S genes, and the species-specific Sequence Characterized Amplified Region (SCAR) markers. All samples from the districts of Ordu province were identified as Meloidogyne incognita by the exact size of 502 bp amplicons by SEC1F/SEC1R scar primers. This study was the first detailed investigation both in the province and country on kiwi fruit.
URI: https://dergipark.org.tr/tr/download/article-file/65244
http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1311
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