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Investigation of the anticancer mechanism of monensin via apoptosis-related factors in SH-SY5Y neuroblastoma cells

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dc.contributor.author Serter Kocoglu, Sema
dc.contributor.author Oy, Ceren
dc.contributor.author Secme, Muecahit
dc.contributor.author Sunay, F. Bahar
dc.date.accessioned 2024-03-26T06:32:09Z
dc.date.available 2024-03-26T06:32:09Z
dc.date.issued 2023
dc.identifier.citation Kocoglu, SS., Oy, C., Secme, M., Sunay, FB. (2023). Investigation of the anticancer mechanism of monensin via apoptosis-related factors in SH-SY5Y neuroblastoma cells. CTS-Clin. Transl. Sci., 16(9), 1725-1735. https://doi.org/10.1111/cts.13593 en_US
dc.identifier.issn 1752-8054
dc.identifier.issn 1752-8062
dc.identifier.uri http://dx.doi.org/10.1111/cts.13593
dc.identifier.uri https://www.webofscience.com/wos/woscc/full-record/WOS:001041901100001
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/5075
dc.description WoS Categories: Medicine, Research & Experimental en_US
dc.description Web of Science Index: Science Citation Index Expanded (SCI-EXPANDED) en_US
dc.description Research Areas: Research & Experimental Medicine en_US
dc.description.abstract Monensin is an ionophore antibiotic that inhibits the growth of cancer cells. The aim of this study was to investigate the apoptosis-mediated anticarcinogenic effects of monensin in SH-SY5Y neuroblastoma cells. The effects of monensin on cell viability, invasion, migration, and colony formation were determined by XTT, matrigel-chamber, wound healing, and colony formation tests, respectively. The effects of monensin on apoptosis were determined by real-time polymerase chain reaction, TUNEL, Western blot, and Annexin V assay. We have shown that monensin suppresses neuroblastoma cell viability, invasion, migration, and colony formation. Moreover, we reported that monensin inhibits cell viability by triggering apoptosis of neuroblastoma cells. Monensin caused apoptosis by increasing caspase-3, 7, 8, and 9 expressions and decreasing Bax and Bcl-2 expressions in neuroblastoma cells. In Annexin V results, the rates of apoptotic cells were found to be 9.66 +/- 0.01% (p < 0.001), 29.28 +/- 0.88% (p < 0.01), and 62.55 +/- 2.36% (p < 0.01) in the 8, 16, and 32 mu M monensin groups, respectively. In TUNEL results, these values were, respectively; 35 +/- 2% (p < 0.001), 34 +/- 0.57% (p < 0.001), and 75 +/- 2.51% (p < 0.001). Our results suggest that monensin may be a safe and effective therapeutic candidate for treating pediatric neuroblastoma. Study Highlights WHAT IS THE CURRENT KNOWLEDGE ON THE TOPIC? Neuroblastoma is the most common extracranial childhood tumor originating from neural crest cells. Neuroblastomas constitute similar to 15% of childhood cancer deaths. There is a need to develop new and alternative advanced treatment approaches for neuroblastoma oncogenesis. Monensin is an ionophore antibiotic with antiparasitic and antibacterial effects. WHAT QUESTION DID THIS STUDY ADDRESS? No study has been found on the anticancer properties of monensin on neuroblastoma cell proliferation, migration, invasion, and apoptosis. This study addresses dose-dependent and apoptotic pathway-mediated anticarcinogenic properties of monensin in neuroblastoma cells in vitro. WHAT DOES THIS STUDY ADD TO OUR KNOWLEDGE? Monensin suppresses neuroblastoma cell proliferation, invasion, migration, and colony formation. Monensin triggers apoptosis by increasing caspase-3, 7, 8, 9, and cleaved-PARP1 expressions and decreasing Bax and Bcl-2 expressions. HOW MIGHT THIS CHANGE CLINICAL PHARMACOLOGY OR TRANSLATIONAL SCIENCE? Monensin may be a safe and effective therapeutic drug candidate in the treatment of pediatric neuroblastoma. en_US
dc.description.sponsorship Scientific and Technological Research Council of Turkey [TUBITAK- SBAG- 120S399] en_US
dc.language.iso eng en_US
dc.publisher WILEY-HOBOKEN en_US
dc.relation.isversionof 10.1111/cts.13593 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject MEDIATED GROWTH-INHIBITION, CYCLE ARREST en_US
dc.title Investigation of the anticancer mechanism of monensin via apoptosis-related factors in SH-SY5Y neuroblastoma cells en_US
dc.type article en_US
dc.relation.journal CTS-CLINICAL AND TRANSLATIONAL SCIENCE en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0002-3180-4007 en_US
dc.contributor.authorID 0000-0002-2231-7979 en_US
dc.identifier.volume 16 en_US
dc.identifier.issue 9 en_US
dc.identifier.startpage 1725 en_US
dc.identifier.endpage 1735 en_US


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