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Characterization of Type VI secretion system in Edwardsiella ictaluri

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dc.contributor.author Kalindamar, Safak
dc.contributor.author Abdelhamed, Hossam
dc.contributor.author Kordon, Adef O.
dc.contributor.author Tekedar, Hasan C.
dc.contributor.author Pinchuk, Lesya
dc.contributor.author Karsi, Attila
dc.date.accessioned 2024-03-20T13:50:57Z
dc.date.available 2024-03-20T13:50:57Z
dc.date.issued 2023
dc.identifier.citation Kalindamar, S., Abdelhamed, H., Kordon, AO., Tekedar, HC., Pinchuk, L., Karsi, A. (2023). Characterization of Type VI secretion system in Edwardsiella ictaluri. PLoS One, 18(12). https://doi.org/10.1371/journal.pone.0296132 en_US
dc.identifier.issn 1932-6203
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0296132
dc.identifier.uri https://www.webofscience.com/wos/woscc/full-record/WOS:001135922100027
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/4829
dc.description WoS Categories: Multidisciplinary Sciences en_US
dc.description Web of Science Index: Science Citation Index Expanded (SCI-EXPANDED) en_US
dc.description Research Areas: Science & Technology - Other Topics en_US
dc.description.abstract Edwardsiella ictaluri is a Gram-negative facultative intracellular fish pathogen causing enteric septicemia of catfish (ESC). While various secretion systems contribute to E. ictaluri virulence, the Type VI secretion system (T6SS) remains poorly understood. In this study, we constructed 13 E. ictaluri T6SS mutants using splicing by overlap extension PCR and characterized them, assessing their uptake and survival in channel catfish (Ictalurus punctatus) peritoneal macrophages, attachment and invasion in channel catfish ovary (CCO) cells, in vitro stress resistance, and virulence and efficacy in channel catfish. Among the mutants, Ei Delta evpA, Ei Delta evpH, Ei Delta evpM, Ei Delta evpN, and Ei Delta evpO exhibited reduced replication inside peritoneal macrophages. Ei Delta evpM, Ei Delta evpN, and Ei Delta evpO showed significantly decreased attachment to CCO cells, while Ei Delta evpN and Ei Delta evpO also displayed reduced invasion of CCO cells (p < 0.05). Overall, T6SS mutants demonstrated enhanced resistance to oxidative and nitrosative stress in the nutrient-rich medium compared to the minimal medium. However, Ei Delta evpA, Ei Delta evpH, Ei Delta evpM, Ei Delta evpN, and Ei Delta evpO were susceptible to oxidative stress in both nutrient-rich and minimal medium. In fish challenges, Ei Delta evpD, Ei Delta evpE, Ei Delta evpG, Ei Delta evpJ, and Ei Delta evpK exhibited attenuation and provided effective protection against E. ictaluri wild-type (EiWT) infection in catfish fingerlings. However, their attenuation and protective efficacy were lower in catfish fry. These findings shed light on the role of the T6SS in E. ictaluri pathogenesis, highlighting its significance in intracellular survival, host cell attachment and invasion, stress resistance, and virulence. The attenuated T6SS mutants hold promise as potential candidates for protective immunization strategies in catfish fingerlings. en_US
dc.description.sponsorship National Institute of Food and Agriculture; Laboratory Animal Resources and Care at the College of Veterinary Medicine en_US
dc.language.iso eng en_US
dc.publisher PUBLIC LIBRARY SCIENCE-SAN FRANCISCO en_US
dc.relation.isversionof 10.1371/journal.pone.0296132 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject PATHOGENIC ESCHERICHIA-COLI, CHANNEL CATFISH, ENTERIC SEPTICEMIA, INTRACELLULAR REPLICATION, VIRULENCE DETERMINANTS, IN-VIVO, SALMONELLA, GENES, PUNCTATUS, IDENTIFICATION en_US
dc.title Characterization of Type VI secretion system in Edwardsiella ictaluri en_US
dc.type article en_US
dc.relation.journal PLOS ONE en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0003-2897-9911 en_US
dc.identifier.volume 18 en_US
dc.identifier.issue 12 en_US


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