dc.contributor.author |
Ozturk, Eylem Akdur |
|
dc.contributor.author |
Manzano-Roman, Raul |
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dc.contributor.author |
Sanchez-Ovejero, Carlos |
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dc.contributor.author |
Caner, Ayse |
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dc.contributor.author |
Angin, Mesude |
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dc.contributor.author |
Gunduz, Cumhur |
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dc.contributor.author |
Karaman, Ulku |
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dc.contributor.author |
Altintas, Nuray |
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dc.contributor.author |
Bozkaya, Halil |
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dc.contributor.author |
Unalp, Omer |
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dc.contributor.author |
Dokumcu, Zafer |
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dc.contributor.author |
Divarci, Emre |
|
dc.contributor.author |
Casulli, Adriano |
|
dc.contributor.author |
Altintas, Nazmiye |
|
dc.contributor.author |
Siles-Lucas, Ma |
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dc.date.accessioned |
2023-01-06T12:17:57Z |
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dc.date.available |
2023-01-06T12:17:57Z |
|
dc.date.issued |
2022 |
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dc.identifier.citation |
Ozturk, EA., Manzano-Roman, R., Sanchez-Ovejero, C., Caner, A., Angin, M., Gunduz, C., Karaman, U., Altintas, N., Bozkaya, H., Unalp, O., Dokumcu, Z., Divarci, E., Casulli, A., Altintas, N., Siles-Lucas, M., Unver, A. (2022). Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis. Acta Tropica, 225, -.Doi:10.1016/j.actatropica.2021.106208 |
en_US |
dc.identifier.isbn |
0001-706X |
|
dc.identifier.isbn |
1873-6254 |
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dc.identifier.uri |
http://dx.doi.org/10.1016/j.actatropica.2021.106208 |
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dc.identifier.uri |
https://www.webofscience.com/wos/woscc/full-record/WOS:000715009000009 |
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dc.identifier.uri |
https://pubmed.ncbi.nlm.nih.gov/34687646 |
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dc.identifier.uri |
http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3678 |
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dc.description |
WoS Categories : Parasitology; Tropical Medicine
Web of Science Index : Science Citation Index Expanded (SCI-EXPANDED)
Research Areas : Parasitology; Tropical Medicine |
en_US |
dc.description.abstract |
The use of serological tests containing multiple immunodominant antigens rather than single antigens have the potential to improve the diagnostic performance in Cystic Echinococcoses (CE) as a complement tool to clear the inconclusive imaging data. Here, we comparatively evaluated the diagnostic value of Hydatid Fluid (HF) and the recently described recombinant multi-epitope antigen DIPOL in IgG-ELISA in a clinically defined cohort of CE patients. The serum samples from 149 CE patients were collected just before surgical or Percutaneous-Aspiration-Injection-Reaspiration (PAIR) procedures. Additionally, serum samples of patients with other parasitic infections (n=49) and healthy individuals (n=21) were also included in the study as controls. To investigate the association between the genotype of the parasite and DIPOL, cyst materials from 20 CE patients were sequenced. In terms of overall sensitivity, HF was higher than DIPOL (82.55%,78.52%, respectively). However, while the sensitivity of HF was higher than DIPOL in patients with active and transitional cysts (83.3%, 75.4%, respectively), sensitivity of DIPOL in inactive cysts was higher compared to HF (95.6%, 78.3%, respectively). The sensitivity of DIPOL depending on cyst stage was statistically significant (P=0.041). In terms of specificity, DIPOL was found to be better than HF (97.71%, 91.43%, respectively). By genotyping, the majority of 20 patients showed G1 genotype (80%). All patients harboring G3 and G1/G3 cyst genotypes were positive with both antigens, while 87.5% of patients with G1 genotype were seropositive with HF and 75% with DIPOL. The overall sensitivity and high specificity of DIPOL suggest that this recombinant protein containing immunodominant epitopes is a potential substitute for the HF by serological tests for the diagnosis of CE. |
en_US |
dc.description.sponsorship |
Funding Orgs : Teaching Staff Training Program; European Commission Seventh Framework Programme (FP7) under the HERACLES project [602051]
Funding Name Preferred : Teaching Staff Training Program; European Commission Seventh Framework Programme (FP7) under the HERACLES project
Funding Text : This study was produced from Eylem Akdur Ozturk's doctoral thesis and supported by Teaching Staff Training Program. The study AC, MSL, RMR and CSO were also supported by the European Commission Sev-enth Framework Programme (FP7) under the HERACLES project |
en_US |
dc.language.iso |
eng |
en_US |
dc.publisher |
ELSEVIER AMSTERDAM |
en_US |
dc.relation.isversionof |
10.1016/j.actatropica.2021.106208 |
en_US |
dc.rights |
info:eu-repo/semantics/openAccess |
en_US |
dc.subject |
SEROLOGICAL DIAGNOSIS; GRANULOSUS INFECTION; SERODIAGNOSIS; PROTEIN; GENE; POLYMORPHISM; FAMILY |
en_US |
dc.subject |
Cystic echinococcosis; Echinococcus granulosus; Enzyme linked immunosorbent assay; Genotype; Multi-epitope recombinant antigen DIPOL; Serodiagnosis |
en_US |
dc.title |
Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis |
en_US |
dc.type |
article |
en_US |
dc.relation.journal |
ACTA TROPICA |
en_US |
dc.contributor.department |
Ordu Üniversitesi |
en_US |
dc.contributor.authorID |
0000-0002-6593-3237 |
en_US |
dc.contributor.authorID |
0000-0001-9183-7591 |
en_US |
dc.contributor.authorID |
0000-0002-2634-4982 |
en_US |
dc.identifier.volume |
225 |
en_US |