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Determination of Sturgeon Species Living in the Black Sea Coasts of Turkey by Sequence, RFLP and Multiplex PCR Analysis Methods

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dc.contributor.author Ciftci, Yilmaz
dc.contributor.author Eroglu, Oguzhan
dc.contributor.author Firidin, Sirin
dc.date.accessioned 2022-09-06T10:30:06Z
dc.date.available 2022-09-06T10:30:06Z
dc.date.issued 2013
dc.identifier.uri http://doi.org/10.36478/javaa.2013.676.682
dc.identifier.uri https://www.researchgate.net/publication/284492286_Determination_of_sturgeon_species_living_in_the_blak_sea_coasts_of_turkey_by_sequence_rflp_and_multiplex_pcr_analysis_methods
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3185
dc.description.abstract In order to perform checks in the trade of fish species under the threat of extinction and with commercial importance such as sturgeon, the fish must be identified rapidly and precisely from any tissue and for this purpose morphometric and protein based determinations can be inadequate. In this study, Acipenser stellatus, Acipenser gueldenstaedtii and Huso huso sampled from Trabzon coasts were used. For RFLP and sequence analysis, the mitochondrial Cytochrome-b (mtDNA Cyt-b) gene was amplified by the help of PCR. From Cyt-b sequence data, it was observed that in the Maximum Parsimony (MP) dendrograrn, 99% of the species were separated. Furthermore in the Cyt-b PCR product restriction enzyme analyses, it was determined that Hinf I and Rsa I enzymes were distinguishing for 3 species. In the multiplex PCR application in the mtDNA D-loop region, the product lengths were determined as 420 bp for A. gueldenstaedtii, 350 bp for H. huso and 260 bp for A. stellatus and they are distinctive for the species. The results showed that the multiplex PCR application is a method which is cheap and effective for the identification of sturgeon species in short duration. en_US
dc.language.iso eng en_US
dc.publisher MEDWELL ONLINEANSINET BUILDING, 308-LASANI TOWN, SARGODHA RD, FAISALABAD 38090, PAKISTAN en_US
dc.relation.isversionof 10.36478/javaa.2013.676.682 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Sturgeon mitochondrial DNA multiplex PCRPCR-RFLP sequence species identification en_US
dc.title Determination of Sturgeon Species Living in the Black Sea Coasts of Turkey by Sequence, RFLP and Multiplex PCR Analysis Methods en_US
dc.type article en_US
dc.relation.journal JOURNAL OF ANIMAL AND VETERINARY ADVANCES en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0003-4553-990X en_US
dc.identifier.volume 12 en_US
dc.identifier.issue 6 en_US
dc.identifier.startpage 676 en_US
dc.identifier.endpage 682 en_US


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