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Clothianidin induces DNA damage and oxidative stress in bronchial epithelial cells

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dc.contributor.author Atli Sekeroglu, Zulal
dc.contributor.author Aydin, Birsen
dc.contributor.author Ilkun, Emre
dc.contributor.author Kontas Yedier, Seval
dc.contributor.author Sekeroglu, Vedat
dc.date.accessioned 2022-08-19T12:20:50Z
dc.date.available 2022-08-19T12:20:50Z
dc.date.issued 2020
dc.identifier.uri http://doi.org/10.1002/em.22376
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/2985
dc.description.abstract Clothianidin (CHN) is a member of the neonicotinoid group of insecticides. Its oxidative and DNA damage potential for human lung cells are not known. Therefore, the present study was designed to examine the effects of CHN on DNA damage and oxidative stress in human bronchial epithelial cells (BEAS-2B) treated with CHN for 24, 72, and 120 hr. Our results indicate that CHN decreased cell viability in a concentration-dependent manner. CHN induced DNA single-strand breaks because alkaline comet parameters such as tail intensity, DNA in the tail, tail moment, and tail length increased. All CHN concentrations also significantly induced the formation of DNA double-strand breaks (DSBs) because it increased phosphorylated H2AX protein foci for all treatment times and p53-binding protein 1 foci for all treatments except for the lowest concentration (0.15 mM) of 120-hr treatment. DNA damage caused by DNA DSBs was not repaired in a 24-hr recovery period. CHN also induced oxidative stress by decreasing reduced glutathione and increasing lipid peroxidation. These results make it necessary to conduct studies about the detailed carcinogenic potential of CHN in humans because it can induce both oxidative and DNA damage. en_US
dc.language.iso eng en_US
dc.publisher WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA en_US
dc.relation.isversionof 10.1002/em.22376 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject bronchial epithelial cells; clothianidin; DNA damage and repair; oxidative stress en_US
dc.subject NEONICOTINOID INSECTICIDE; EXPOSURE; GAMMA-H2AX; 53BP1; ASSAY en_US
dc.title Clothianidin induces DNA damage and oxidative stress in bronchial epithelial cells en_US
dc.type article en_US
dc.relation.journal ENVIRONMENTAL AND MOLECULAR MUTAGENESIS en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0002-3552-3819 en_US
dc.contributor.authorID 0000-0003-3532-573X en_US
dc.identifier.volume 61 en_US
dc.identifier.issue 6 en_US
dc.identifier.startpage 647 en_US
dc.identifier.endpage 655 en_US


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