DSpace Repository

A Multicenter Evaluation of Blood Culture Practices, Contamination Rates, and the Distribution of Causative Bacteria

Show simple item record

dc.contributor.author Aksaray, Sebahat
dc.contributor.author Altindis, Mustafa
dc.contributor.author Atasoy, Ali Riza
dc.contributor.author Cetinkol, Yeliz
dc.contributor.author Cicek, Aysegul Copur
dc.contributor.author Dal, Tuba
dc.contributor.author Demiray, Tayfur
dc.contributor.author Demirkan, Idris
dc.contributor.author Dogan, Metin
dc.contributor.author Ece, Gulfem
dc.contributor.author Guducuoglu, Huseyin
dc.contributor.author Gulhan, Baris
dc.contributor.author Gultepe, Bilge Sumbul
dc.contributor.author Iraz, Meryem
dc.contributor.author Karabay, Oguz
dc.contributor.author Kaya, Selcuk
dc.contributor.author Kilic, Abdullah
dc.contributor.author Kocoglu, Esra
dc.contributor.author Koroglu, Mehmet
dc.contributor.author Ozdemir, Mehmet
dc.contributor.author Sahin, Idris
dc.contributor.author Sengil, Ahmet Zeki
dc.contributor.author Temiz, Hakan
dc.date.accessioned 2022-08-16T11:59:28Z
dc.date.available 2022-08-16T11:59:28Z
dc.date.issued 2016
dc.identifier.uri http://doi.org/10.5812/jjm.29766
dc.identifier.uri https://brief.land/jjm/articles/56612.html
dc.identifier.uri http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/2075
dc.description.abstract Background: The prognostic value of blood culture testing in the diagnosis of bacteremia is limited by contamination. Objectives: In this multicenter study, the aim was to evaluate the contamination rates of blood cultures as well as the parameters that affect the culture results. Materials and Methods: Sample collection practices and culture data obtained from 16 university/research hospitals were retrospectively evaluated. A total of 214,340 blood samples from 43,254 patients admitted to the centers in 2013 were included in this study. The blood culture results were evaluated based on the three phases of laboratory testing: the pre-analytic, the analytic, and the post-analytic phase. Results: Blood samples were obtained from the patients through either the peripheral venous route (64%) or an intravascular catheter (36%). Povidone-iodine (60%) or alcohol (40%) was applied to disinfect the skin. Of the 16 centers, 62.5% have no dedicated phlebotomy team, 68.7% employed a blood culture system, 86.7% conducted additional studies with pediatric bottles, and 43.7% with anaerobic bottles. One center maintained a blood culture quality control study. The average growth rate in the bottles of blood cultures during the defined period (1259 -26,400/year) was 32.3%. Of the growing microorganisms, 67% were causative agents, while 33% were contaminants. The contamination rates of the centers ranged from 1% to 17%. The average growth time for the causative bacteria was 21.4 hours, while it was 36.3 hours for the contaminant bacteria. The most commonly isolated pathogens were Escherichia coli (22.45%) and coagulase-negative staphylococci (CoNS) (20.11%). Further, the most frequently identified contaminant bacteria were CoNS (44.04%). Conclusions: The high contamination rates were remarkable in this study. We suggest that the hospitals' staff should be better trained in blood sample collection and processing. Sterile glove usage, alcohol usage for disinfection, the presence of a phlebotomy team, and quality control studies may all contribute to decreasing the contamination rates. Health policy makers should therefore provide the necessary financial support to obtain the required materials and equipment. en_US
dc.language.iso eng en_US
dc.publisher AHVAZ JUNDISHAPUR UNIV MED SCI, PO BOX 6357-33118, AHVAZ, 00000, IRAN en_US
dc.relation.isversionof 10.5812/jjm.29766 en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Blood Specimen Collection; Phlebotomy; Blood-Borne Pathogens; Bacteriological Techniques en_US
dc.subject INFECTIOUS-DISEASES SOCIETY; INTENSIVE-CARE-UNIT; LABORATORY MEDICINE; DIAGNOSIS; WORKLOAD; AMERICA; TRENDS en_US
dc.title A Multicenter Evaluation of Blood Culture Practices, Contamination Rates, and the Distribution of Causative Bacteria en_US
dc.type article en_US
dc.relation.journal JUNDISHAPUR JOURNAL OF MICROBIOLOGY en_US
dc.contributor.department Ordu Üniversitesi en_US
dc.contributor.authorID 0000-0002-0552-1337 en_US
dc.contributor.authorID 0000-0002-2605-1282 en_US
dc.contributor.authorID 0000-0002-9316-771X en_US
dc.contributor.authorID 0000-0003-0411-9669 en_US
dc.contributor.authorID 0000-0003-0502-432X en_US
dc.contributor.authorID 0000-0003-1514-1685 en_US
dc.contributor.authorID 0000-0003-4940-4498 en_US
dc.identifier.volume 9 en_US
dc.identifier.issue 1 en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account