Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/5114
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dc.contributor.authorSelimovic, Amina-
dc.contributor.authorKara, Goknur-
dc.contributor.authorDenkbas, Emir Baki-
dc.date.accessioned2024-03-26T06:36:57Z-
dc.date.available2024-03-26T06:36:57Z-
dc.date.issued2022-
dc.identifier.citationSelimovic, A., Kara, G., Denkbas, EB. (2022). Magnetic gelatin nanoparticles as a biocompatible carrier system for small interfering RNA in human colorectal cancer: Synthesis, optimization, characterization, and cell viability studies. Mater. Today Commun., 33. https://doi.org/10.1016/j.mtcomm.2022.104616en_US
dc.identifier.issn2352-4928-
dc.identifier.urihttp://dx.doi.org/10.1016/j.mtcomm.2022.104616-
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000933379000004-
dc.identifier.urihttp://earsiv.odu.edu.tr:8080/xmlui/handle/11489/5114-
dc.descriptionWoS Categories: Materials Science, Multidisciplinaryen_US
dc.descriptionWeb of Science Index: Science Citation Index Expanded (SCI-EXPANDED)en_US
dc.descriptionResearch Areas: Materials Scienceen_US
dc.description.abstractIron oxide-based nanoparticles have gained tremendous attention in developing next-generation personalized medicine modalities. Gelatin can be a good alternative for encapsulating iron oxide nanoparticles with its biocompatibility, biodegradability, low immunogenicity, and richness of functional groups. Herein, magnetic iron oxide nanoparticles (MNPs) were synthesized, coated with gelatin (Gel-MNPs), and loaded with mammalian target of rapamycin (mTOR)-silencing siRNA to induce the in vitro therapeutic effect in colorectal cancer (CRC) cells. To the best of our knowledge, this study is the first report using Gel-MNPs as siRNA carriers. We first optimized several experimental conditions for the preparation of MNPs and Gel-MNPs and the resulting opti-mized nanoparticles showed a narrow size and size distribution. Gelatin-coating increased the storage stability by preventing the aggregation of MNPs and did not alter the magnetic characteristics of MNPs significantly. siRNA encapsulation abilities of Gel-MNPs were determined in the range of 18.4% and 41.5% in varying siRNA amounts. Bare Gel-MNPs were highly cytocompatible against CRC cells, Caco-2, while Gel-MNPs-mTOR-siRNA exhibited a significant anticancer effect to kill these cells. Comparison with HiPerFect, a commercial siRNA transfection reagent, demonstrated that Gel-MNPs-mTOR-siRNA inhibited cell viability almost similar to or better than HiPerFect-mTOR-siRNA. Taken together, our data indicated that Gel-MNPs could potentially be used in further gene silencing approaches as a safe and multifunctional siRNA carrier candidate.en_US
dc.language.isoengen_US
dc.publisherELSEVIER-AMSTERDAMen_US
dc.relation.isversionof10.1016/j.mtcomm.2022.104616en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIron oxide nanoparticles, Gelatin, SiRNA, Nanocarriers, Gene silencing, Cancer therapyen_US
dc.subjectIRON-OXIDE NANOPARTICLES, IN-VITRO, SIRNA DELIVERY, 2-STEP DESOLVATION, DRUG-DELIVERY, GENE, SIZE, COPRECIPITATION, ENCAPSULATION, NANOCARRIERSen_US
dc.titleMagnetic gelatin nanoparticles as a biocompatible carrier system for small interfering RNA in human colorectal cancer: Synthesis, optimization, characterization, and cell viability studiesen_US
dc.typearticleen_US
dc.relation.journalMATERIALS TODAY COMMUNICATIONSen_US
dc.contributor.departmentOrdu Üniversitesien_US
dc.identifier.volume33en_US
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