In vitro propagation in different media of some cherry genotypes as candidate rootstocks for cherry

Abstract

In this study, possibilities of in vitro production of cherry and sour cherry genotypes, candidate rootstocks, were investigated. The study was carried out in the tissue culture laboratory of Aksa Agriculture, Turkey. The lateral and top buds of annual shoots were used as explants. The buds were taken at different times of the year and the media used were MS (Murashige and Skoog), WPM (Woody Plant Medium) and QL (Quoirin and Lepoivre), supplied with 0.1 mg L-1 IBA, 0.1 mg L-1 GA(3), 1.0 mg L-1 BAP. During the propagation phase, explants were placed in growth chambers under a 16 h photoperiod, with a light intensity of 41 mol m(-2) s(-1), and temperature of 25 +/- 1 degrees C. Explants were subcultured for 4 weeks. Contamination rates in explants taken in June were lower. In the genotypes candidate for cherry and sour cherry rootstocks, the MS medium has a higher degree of multiplication rate.