Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/4402
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dc.contributor.authorGursoy, Sultan-
dc.contributor.authorDukar, Nilgun-
dc.contributor.authorYaman, Yesim Tugce-
dc.contributor.authorAbaci, Serdar-
dc.contributor.authorKuralay, Filiz-
dc.date.accessioned2024-03-15T11:09:02Z-
dc.date.available2024-03-15T11:09:02Z-
dc.date.issued2019-
dc.identifier.citationGürsoy, S., Dükar, N., Yaman, YT., Abaci, S., Kuralay, F. (2019). Electroactive polyglycine coatings for nanobiosensing applications: Label-free DNA hybridization, DNA-Antitumor agent interaction and antitumor agent determination. Anal. Chim. Acta, 1072, 15-24. https://doi.org/10.1016/j.aca.2019.04.044en_US
dc.identifier.issn0003-2670-
dc.identifier.issn1873-4324-
dc.identifier.urihttp://dx.doi.org/10.1016/j.aca.2019.04.044-
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000469021200002-
dc.identifier.urihttp://earsiv.odu.edu.tr:8080/xmlui/handle/11489/4402-
dc.descriptionWoS Categories: Chemistry, Analyticalen_US
dc.descriptionWeb of Science Index: Science Citation Index Expanded (SCI-EXPANDED)en_US
dc.descriptionResearch Areas: Chemistryen_US
dc.description.abstractBioinspired materials have attracted great attention due to their great functionality, bioactivity and biocompability. In particular, electroactive polyamino acid surfaces endow preparation of robust coatings for various applications. In this research article, preparation of carbon nanotubes doped polyglycine coated electrodes and their applications in the biomedical field such as DNA hybridization, DNA-antitumor agent interaction and antitumor agent determination were described. This biosensing platform was created using a simple, reproducible and fast in situ and one-pot electropolymerization procedure onto graphite surfaces by cyclic voltammetry. The coated electrodes were characterized with cyclic voltammetry and electrochemical impedance spectroscopy (EIS). After the characterization studies, bioapplications of the proposed electrode were demonstrated. The new electrode led to significant improvement for the investigation of the electrochemical behavior of double-stranded DNA (dsDNA). 6-fold and 5-fold improvements were obtained for oxidation of the electroactive DNA bases, guanine (G) and adenine (A), respectively over the bare electrode. For these steps, each electrode was characterized with scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Then, DNA hybridization studies were performed in the light of these results. The proposed electrode allowed the quantification of specific target DNA down to 11.2 fM in serum samples (n = 3). In addition, it constituted a sensitive `biosensing platform for electrochemical monitoring of the interaction between dsDNA and a commonly used antitumor agent, Mitomycin C. Mitomycin C determination was also carried out using the inhibition effect occurred at the guanine oxidation signal. The detection limit of this antitumor agent was found as 1.78 mg L-1 in untreated serum samples (n = 3). (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherELSEVIER SCIENCE BV-AMSTERDAMen_US
dc.relation.isversionof10.1016/j.aca.2019.04.044en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPolyglycine, Carbon nanotubes, DNA hybridization, Antitumor agent, DNA-Antitumor agent interactionen_US
dc.subjectCARBON-PASTE ELECTRODE, ELECTROCHEMICAL DETERMINATION, ACID BIOSENSOR, ASCORBIC-ACID, DOPAMINE, SENSOR, GENEen_US
dc.titleElectroactive polyglycine coatings for nanobiosensing applications: Label-free DNA hybridization, DNA-Antitumor agent interaction and antitumor agent determinationen_US
dc.typearticleen_US
dc.relation.journalANALYTICA CHIMICA ACTAen_US
dc.contributor.departmentOrdu Üniversitesien_US
dc.contributor.authorID0000-0003-0356-9692en_US
dc.identifier.volume1072en_US
dc.identifier.startpage15en_US
dc.identifier.endpage24en_US
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