Antipyrine derived-Schiff base copper complex: Synthesis, characterization, and in vitro evaluation

Abstract

The copper(II) complex (NMPAAP-Cu) of 1,5-dimethyl-4-(((1-methyl-1H-pyrrol-2-yl)methylene)amino)-2phenyl-1,2-dihydro-3H-pyrazole-3-one (NMPAAP) was synthesized and analyzed by elemental analysis, magnetic susceptibility molar conductivity, thermal analysis, and defined spectral features using MS, IR, UV-vis, 1H, 13C NMR. In vitro biological activity of the complex was also investigated. Antimicrobial potency was evaluated by Disc Diffusion Method. Antioxidant activity was investigated by using DPPH radical scavenging and metal chelating activity tests. The complex exhibited a dose-dependent increase in its antioxidant capacity. The in vitro cytotoxic and anticancer potentials were evaluated in the human breast cancer cell line MCF-7. Data showed that NMPAAP-Cu significantly decreased the cell viability of MCF-7 cells in a concentration-dependent manner. AO/ EB staining showed that the complex significantly increased apoptosis in MCF-7 cells. 50 mu M NMPAAP-Cu also inhibited the migration of MCF-7 cells revealed in the wound healing assay. The study results indicated that the NMPAAP-Cu showed very high anticancer activity against MCF-7 carcinoma cells.