Effect of Different Salicylic Acid Doses on Kober 5 BB Grapevine Rootstock in Vitro Drought Stress Conditions

Abstract

5 BB grapevine rootstock cuttings were utilized to create the explants used in the experiment. The one-node micro-cuttings were used in the experiment. The sterilized explants were planted in Murashige and Skoog (MS) basic nutrient medium with 1 mg L-1 6-benzylaminopurine (BAP). After 2 weeks of planting, the shoots were transferred to MS nutrient medium in which 1 mg L-1 indole-3-butyric acid (IBA) for rooting and elongation of shoot. After 3 weeks, the shoots were obtained by cutting 2-3 cm from the top of the elongated shoots and were transferred to MS medium containing 1 mg L-1 IBA containing different doses of sorbitol (0 M, 0.2 M, 0.4 M) and salicylic acid (SA) (0 mM, 0.5 mM, 1 mM and 2 mM) to examine their rooting and drought responses. After drought and different doses of SA applications, some parameters of shoot growth and physiological features were determined. According to the overall findings of the evaluated properties, the 0.2 M dose of sorbitol produced more effective drought stress in this study as compared with the other tested doses. According to this study, adding 2 mM SA to the nutritive medium had a positive impact on reducing the adverse effects of drought stress, degree of damage (1-4), tolerance rate, ion flux (%), rate of cell membrane damage (%), and proportional water content (%). It was determined that the negative effect of drought stress on the plants was reduced with the application of 1 and 2 mM SA and the highest sorbitol dose.