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http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/4045
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DC Field | Value | Language |
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dc.contributor.author | Caldas, Alev | - |
dc.contributor.author | Boercek Kasurka, Ceren | - |
dc.contributor.author | Erturk, Oemer | - |
dc.date.accessioned | 2024-03-15T06:53:29Z | - |
dc.date.available | 2024-03-15T06:53:29Z | - |
dc.date.issued | 2024 | - |
dc.identifier.citation | Çaldas, A., Kasurka, CB., Ertürk, O. (2024). Effects of wasp (Vespa crabro) nest extracts on virus replication of Autographa californica nuclear polyhedrosis virus on Spodoptera frugiperda cell culture. Cytotechnology, 76(1), 123-137. https://doi.org/10.1007/s10616-023-00603-0 | en_US |
dc.identifier.issn | 0920-9069 | - |
dc.identifier.issn | 1573-0778 | - |
dc.identifier.uri | http://dx.doi.org/10.1007/s10616-023-00603-0 | - |
dc.identifier.uri | https://www.webofscience.com/wos/woscc/full-record/WOS:001106624000002 | - |
dc.identifier.uri | http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/4045 | - |
dc.description | WoS Categories: Biotechnology & Applied Microbiology; Cell Biology | en_US |
dc.description | Web of Science Index: Science Citation Index Expanded (SCI-EXPANDED) | en_US |
dc.description | Research Areas: Biotechnology & Applied Microbiology; Cell Biology | en_US |
dc.description.abstract | The antiviral properties of the extracts of Vespa crabro nests collected from the Black Sea, Turkey have been investigated on Spodoptera frugiperda (Sf) cell cultures of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV). The effect of nests on cell viability and cytotoxicity analysis and the antiviral assay was studied, and the cytopathic effects of the virus were detected. The nest's viral content was identified. The impact of nest extracts on the protein synthesis of the virus was investigated. Also interaction with pUC18 plasmid DNA was investigated, to analyse the protective effects of the Vespa crabro nest extract againist to hydroxyl radical-mediated DNA damage. 50 mu g/ml concentration of ethanol, acetone, and petroleum ether extracts of the nests reduced the cytopathic effects of baculovirus on Sf cells. The extracts delayed infection above 25 mu g/ml concentration. When the effects of nest extracts on virus titer were evaluated; the 50 mu g/ml concentration of the acetone extract of the nest showed the highest effect (75%) reducing the virus titer. 25 mu g/ml concentration of the ethanol extract of the nest showed the lowest effect (33.33%) with a reduction. The presence of polyhedrin protein was observed at 25 mu g/ml concentrations of acetone and petroleum ether extracts. When the potential of the nest extracts to repair DNA damage, the nest extracts were found to have a concentration-dependent repair feature in different applications. As a result of bioactive component analysis, (Z) 9-Tricosane and (cis)-2-nonadecene (1.65%) were found to have the highest % areas. In other respects, 1H-Purine-6-amine, 2-dodecanol and hexadecanoic acid compaunds were Additionally, 1H-Purine-6-amine, 2-dodecanol and hexadecanoic acid compounds, which are associated with antiviral activity, also determined in the biocomponent analysis. | en_US |
dc.description.sponsorship | Ordu University [B-1817] | en_US |
dc.language.iso | eng | en_US |
dc.publisher | SPRINGER-DORDRECHT | en_US |
dc.relation.isversionof | 10.1007/s10616-023-00603-0 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Sf 9 cell line, Vespa crabro nests, Autographa californica multicapsid nuclear polyhedrosis virus, Cytotoxicity, Bioactive component | en_US |
dc.subject | RECOMBINANT BACULOVIRUSES, VESPIDAE HYMENOPTERA, INFECTION, TOXICITY, PROTEINS, ACULEATA, REGIONS | en_US |
dc.title | Effects of wasp (Vespa crabro) nest extracts on virus replication of Autographa californica nuclear polyhedrosis virus on Spodoptera frugiperda cell culture | en_US |
dc.type | article | en_US |
dc.relation.journal | CYTOTECHNOLOGY | en_US |
dc.contributor.department | Ordu Üniversitesi | en_US |
dc.identifier.volume | 76 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 123 | en_US |
dc.identifier.endpage | 137 | en_US |
Appears in Collections: | Moleküler Biyoloji ve Genetik Bölümü |
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