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Title: | Occurency of Giardia duodenalis assemblages in river water sources of Black Sea, Turkey |
Authors: | Karanis, Panagiotis Koloren, Zeynep Seferoglu, Onuralp Ordu Üniversitesi 0000-0001-9708-2716 0000-0002-0733-0470 |
Keywords: | Giardia duodenalis; PCR; LAMP; Assemblages A and B; River water; Giresun; Samsun; Turkey WASTE-WATER; MOLECULAR CHARACTERIZATION; PROTOZOAN PARASITES; NESTED PCR; CRYPTOSPORIDIUM; GENOTYPES; TRANSMISSION; SUPPLIES; IDENTIFICATION; EPIDEMIOLOGY |
Issue Date: | 2016 |
Publisher: | ELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS |
Abstract: | A total of 420 environmental water samples and 120 drinking water samples from 45 different sampling sites of the Black Sea in Turkey were collected between 2012 and 2014. Genomic DNA was isolated from all the investigated water samples and comparativelly analyzed by Loop-mediated isothermal amplification (LAMP) of the elongation factor 1 Alfa (EF1 alpha) gene, and by nested Polymerase Chain Reaction (nPCR) of the small subunit (SSU) rRNA and semi-nested PCR (snPCR) of the glutamate dehydrogenase gene (GDH). 141 (58.7%), 125 (52.1%) and 120 (50%) samples respectivelly were positive by each method. Out of 240 environmental samples collected from 25 sites of Samsun Province have been found positive for G. duodenalis by LAMP, nPCR and snPCR, respectively. 55 (30.5%), 50 (27.8%) and 47 (26.1%) of 180 environmental samples collected from 20 other sampling sites of Giresun Province were positive for Giardia by LAMP, nPCR and snPCR, respectively. Five PCR products from different samples of the Giresun Province and 10 other samples from the Samsun Province were found positive for G. duodenalis assemblage B. Five PCR products from Giresun Province and 5 samples from Samsun Province were found positive for G. duodenalis assemblage A. This is the first report about G. duodenalis assemblages A and B from water samples investigations in Black Sea of Turkey. (C) 2016 Elsevier B.V. All rights reserved. |
URI: | http://doi.org/10.1016/j.actatropica.2016.09.025 https://www.sciencedirect.com/science/article/pii/S0001706X16305575?via%3Dihub http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/3004 |
Appears in Collections: | Moleküler Biyoloji ve Genetik Bölümü |
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