Please use this identifier to cite or link to this item: http://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1486
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dc.contributor.authorEulenburg, Volker-
dc.contributor.authorHeinlein, Anja-
dc.contributor.authorPischetsrieder, Monika-
dc.contributor.authorSahin, Suemeyye-
dc.contributor.authorVillmann, Carmen-
dc.date.accessioned2022-08-16T05:06:33Z-
dc.date.available2022-08-16T05:06:33Z-
dc.date.issued2017-
dc.identifier.urihttp://doi.org/10.1016/j.jff.2017.08.033-
dc.identifier.urihttp://earsiv.odu.edu.tr:8080/xmlui/handle/11489/1486-
dc.description.abstractThe spice Syzygium aromaticum L. (clove buds) exerts topical anesthetic and analgesic effects. Since GABA(A) receptors are an emerging drug target for pain treatment, the effects of aqueous clove extracts on the human alpha 1 beta 2-GABA(A) receptor were tested by two-electrode voltage clamp technique applying a three-step test system. The extract significantly and specifically potentiated the GABA-induced currents by an allosteric mechanism in concentration-dependent manner (0.5-5 mu g/mL up to 426 +/- 23%) HPLC-based activity-guided fractionation revealed eugenol as main determinant of this GABAergic activity. Acetyleugenol, an important component of clove bud oil, showed even higher activity than eugenol (1 mu g/mL; 308 +/- 26% versus 234 +/- 29%), but was detected in the aqueous extract only in trace amounts. Thus, the analgesic effects of clove might be partially mediated by positive modulation of the GABAA receptor and eugenol is a major contributor to this activity. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.language.isoengen_US
dc.publisherELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSen_US
dc.relation.isversionof10.1016/j.jff.2017.08.033en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcetyleugenol; Analgesic activity; Clove buds; Eugenol; GABA(A) receptor; Two-electrode voltage clamp techniqueen_US
dc.subjectRECOMBINANT GABA(A) RECEPTORS; GATED ION CHANNELS; XENOPUS OOCYTES; ESSENTIAL OIL; GENERAL-ANESTHETICS; MODULATION; POTENTIATION; METHYLEUGENOL; ALCOHOL; THYMOLen_US
dc.titleIdentification of eugenol as the major determinant of GABA(A)-receptor activation by aqueous Syzygium aromaticum L. (clove buds) extracten_US
dc.typearticleen_US
dc.relation.journalJOURNAL OF FUNCTIONAL FOODSen_US
dc.contributor.departmentOrdu Üniversitesien_US
dc.contributor.authorID0000-0002-4878-5746en_US
dc.identifier.volume37en_US
dc.identifier.startpage641en_US
dc.identifier.endpage649en_US
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